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CorrelationQuestion
Is It Really Good to Detect E. coli By Enzyme Substrate Method?
Date:2024-10-29 Browse: 2

In fact, we have learned before that the method of monitoring Escherichia coli in water has multi-tube fermentation, filter membrane method, enzyme substrate and other methods, of which the enzyme substrate method is a more advanced method, then the enzyme substrate method to detect Escherichia coli is really good?

The Ddvantages of Enzyme Substrate Method Compared With Multi-tube Fermentation and Filtration Membrane Method.

Water colibacillus enzyme substrate test system

It consists of LK program-controlled quantitative sealing machine, 51 or 97 hole quantitative testing disc, 100ml quantitative bottle and enzyme substrate detection reagent. Test water sample range: drinking water, source water, waste water, food water, medical water, etc.

Enzyme substrate method is GB5750-2006 included for E. coli detection standard method, enzyme substrate method is currently the advanced method for the detection of E. coli in water, at present, with its convenient and fast, low false positive, suitable for rapid detection of a large number of samples and other advantages are gradually recognized by the domestic testing department.

Compared with the multi-tube fermentation and filtration membrane method, the enzyme substrate method has many fewer detection steps, and the requirements for water environment monitoring are not high, and the detection time can be as little as 24 hours, which has a good application prospect in daily water sample monitoring and emergency monitoring, and can timely detect, prevent and reduce the incidence of major public safety accidents.

 

Method advantages

· No need to operate in A sterile room;

· Manual operation time is less than one minute;

· No need for medium preparation and sterilization of large quantities of glassware;

· Qualitative and quantitative analysis can be completed in 24 hours, without verification test;

It has been included in: GB/T5750.12-2006 “Standard Test Method for Drinking Water” microbial indicators.

 

The WECT-900 Escherichia coli online analyzer produced by Hangzhou Modi adopts the enzyme substrate method, the principle that the enzyme and the light signal after the reaction of bacterial culture are proportional to the change, reflecting the total amount of bacteria or coliform bacteria in the sample. Escherichia coli test technology is a kind of Escherichia coli detection system based on biosensor technology, which provides an effective detection means to deal with water supply contamination. The number of E. coli in water samples can be expressed by comparing the degree of light intensity weakening of the solution with that of a non-toxic control blank experiment.

 

Features of the product

· Simple operation, integrated report generation, real-time monitoring of the whole process of reaction, and dynamic display of the reaction curve of the test project;

· Automatic quality control and calibration to ensure the consistency and reliability of test results;

· Automatic control, user programmable detection parameters, to adapt to different applications for suspicious samples automatically start the cycle detection mode, at the same time start the control center alarm machine after power call again, automatically resume the working state;

· Reliable operation, low cost of use and maintenance, automatic completion of sample and reagent dispensing, mixing and cleaning, avoid cross contamination easy maintenance, low operating cost, small reagent consumption.