In fact, when it comes to detecting coliform bacteria in water, it is a common and indispensable indicator in the Medical Wastewater Online Monitoring. In this article, we will jointly familiarize ourselves with the relevant content of coliform bacteria monitoring in water.

1.What is the purpose of detecting coliform bacteria in water? The main purpose is to understand the relationship between the total amount of coliform bacteria and the water quality conditions. There are many detection methods. This WECT-900 Coliform Online Analyzer uses the enzyme substrate method.

2.How to operate it? It can be accomplished through the following steps.

1) Water sample collection

· If it is tap water, you can sterilize it by burning the tap with a flame for 3 minutes, then unscrew the tap and let the water flow for 5 minutes to allow stagnant water to accumulate in the drain pipe. Then, use a sterilized bottle to take a water sample for easier testing.

· What if it’s pool water, lake water or river water? Take a sterile small-mouthed bottle with a glass stopper, immerse it in a water layer 10 to 15cm deep from the water surface, with the bottle mouth facing upwards. Remove the stopper. Once the bottle is filled with water, close the cap. After taking it out, test it immediately or store it in the refrigerator for no more than 24 hours.

2) Detection of coliform bacteria by enzyme substrate method.

This method is to detect the content of coliform bacteria in water by using the principle that the reaction between enzymes and bacterial cultures causes the changes in their light signals to be proportional. This technical approach is a coliform bacteria detection system based on biosensing technology. Simply put, it is actually an effective means of detecting water supply pollution.

3) What are the main issues that need to be addressed during the detection process?

· carefully prepares different types of culture media;

· During the detection process, b should reasonably control the amount of water sample added.

· carefully selects typical colonies of coliform bacteria.